Four microliters of the polyadenylated RNA were used in a 20 µL reverse transcription reaction with a poly T adaptor primer. M.; Looijen-Salamon, Monika G.; de Geus-Oei, Lioe-Fee; van der Drift, Miep A.; van der Heijden, Erik H. M.; Oyen, Wim J.; Visser, Eric P.; Span, Paul N.; Bussink, Johan.One microliter of the reverse transcription was used in a 20 µL q PCR reaction with primers against the human micro RNAs ( He La cells, 100 µL rat blood and 10 mg hamster kidney using Norgen's Total RNA Purification Kit. Teresa Lorenzi, Elena Annabel Niţulescu, Antonio Zizzi, Maria Lorenzi, Francesca Paolinelli, Simone Domenico Aspriello, Monica Baniţă, Ştefania Crăiţoiu, Gaia Goteri, Giorgio Barbatelli, Tommaso Lombardi, Roberto Di Felice, Daniela Marzioni mail, Corrado Rubini, Mario Castellucci.RT-PCR was performed according to Shi and Chiang (2005).
One hundred nanograms of extracted RNA from each kit was applied to an Illumina micro RNA expression profiling kit. Norgen's Total RNA Purification Kit isolates mi RNA from plasma with better diversity than a leading competitor. Scatter plots display better consistency (better clustering) of replicate signals from Norgen's samples. Total RNA including mi RNA was isolated from 100 µL of plasma using Norgen's Total RNA Purification Kit or 625 µL of plasma using Competitor A's leading mi RNA Kit, and was applied to an NCode expression profiling kit. Norgen's Total RNA Purification Kit isolates the complete size range of RNA, including small RNAs without the use of phenol.Total RNA was isolated from 0.75 million He La cells using Norgen's Total RNA Purification Kit, various silica-based competitors and phenol-based TRI Reagent.In brief, one microliter of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using mi R-21 stem-loop reverse primer or oligo d T primer.
Two microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human Figure 6. Tarrand, Zeev Estrov, Milan Radovich, Han Liang, Massimo Negrini, Thomas J. PCR products of beta-actin were detected from as little as a single cell. Linear and Sensitive Isolation of Both Large and Small RNA. Hill, Michael Milosevic, Fei-Fei Liu Katerina Tomankova, Katerina Polakova, Klara Pizova, Svatopluk Binder, Marketa Havrdova, Mary Kolarova, Eva Kriegova, Jana Zapletalova, Lukas Malina, Jana Horakova, Jakub Malohlava, Argiris Kolokithas-Ntoukas, Aristides Bakandritsos, Hana Kolarova, Radek Zboril Peter L. Norgens Total RNA Purification Kit allows consistent isolation of both large and small RNA from different input amounts. Clarke, Philip Wong, Shaoming Yin, Rui Yan, Daryl Waggott, Paul C. Pontoppidan, Karina Jordan, Anting Liu Carlsen, Hilde Hylland Uhlving, Katrine Kielsen, Mette Christensen, Marianne Ifversen, Claus Henrik Nielsen, Per Sangild, Niels Henrik Helweg Heegaard, Carsten Heilmann, Henrik Sengeløv, Klaus Müller. Total RNA was isolated from 1 x 10 cells using Norgens Total RNA Purification Kit and a competitors kit.Five microliters and 1 µL of the 50 µL isolated RNA was analyzed on an agarose gel (Panel A) and the Agilent® 2100 Bio Analyzer RNA Nano 6000 chip (Panel B), respectively.Norgen's Total RNA Purification Kit consistently isolates high quality RNA from various inputs that score a RIN value between 8 and 10.]" Figure 4. Norgen's Total RNA Purification Kit allows sensitive RNA extraction from as little as a single cell. Cui, Kerry-Anne Rye, Praveen Sethupathy, and Alan T.